Figure 5 | Scientific Reports

Figure 5

From: Urban Particles Elevated Streptococcus pneumoniae Biofilms, Colonization of the Human Middle Ear Epithelial Cells, Mouse Nasopharynx and Transit to the Middle Ear and Lungs

Figure 5

HMEECs viability, apoptosis and reactive oxygen species production in presence of UP (200 µg/ml) or S. pneumoniae (MOI 10) or Co-treatment. (a) HMEECs viability upon UP or S. pneumoniae or Co-treatment. (b) Apoptosis detection using flow cytometry analysis of Annexin V/PI stained HMEECs treated with UP only or Streptococcus pneumoniae only or co-treatment (UP + Streptococcus pneumoniae. (c) Graphical representation of HMEECs percentage showed apoptosis. (d) ROS production detection in HMEEC treated with UP or Streptococcus pneumoniae or co-treatment. The error bars representing standard deviation from mean. The statistically significant were calculated by one-way ANOVA test and p-value less than 0.05 were considered significant.

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