Figure 2
Schematic workflow of the experimental design. The resazurin viability assay was used to evaluate drug response using two breast cancer cell lines (MCF7 and HCC38) and three pharmaceutical drugs (bortezomib, carboplatin and cisplatin). The assay was optimized using Analysis of Variance (ANOVA) to identify potential experimental confounders and Z-factor (Z), Signal window (SW) and coefficient of variation (CV) to evaluate the signal dynamic range. Thereafter, the optimized parameters were implemented using four breast cancer cell lines (MCF7, HCC38, MCF-10A, and MDA-MB-436) and three pharmaceutical drugs (bortezomib, carboplatin and cisplatin). Finally, our data were compared to published data (pharmacoDB42 and Hafner et al.31).
