Figure 6
Cell viability data generated after optimization of the resazurin reduction assay. (a) Cell viability in MCF7, HCC38, MCF-10A, and MDA-MB-436 breast cancer cells treated with 1–10,000 nM bortezomib for 24 hours. Three independent experiments were performed in triplicate. Error bars depict the standard error of the mean. (b) Proteasome activity in MCF7, HCC38, MCF-10A, and MDA-MB-436 cells determined after two hours of bortezomib exposure. T-test was used to calculate statistical significance (Benjamini-Hochberg adjusted p-values). ns = not significant (P > 0.05); *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001. (c) Bortezomib induces G2/M arrest in HCC38 cells treated with 5 nM bortezomib for 24 hours. The dose-response curves and bar plots were generated using ggplot2 (version 3.2.1) in R51. The cell cycle distribution graphs were generated using the Flowing Software (http://flowingsoftware.btk.fi/index.php?page=1).
