Figure 8
Growth rate inhibition data generated after optimization of the resazurin reduction assay. (a) Drug potency and efficiency was determined on MCF7, HCC38, MCF-10A, and MDA-MB-436 cell lines using growth rate inhibition metrics (GR50 and GRmax). Three independent experiments were performed in triplicate. Error bars depict the standard error of the mean. (b) Comparison of GR50 and (c) GRmax values for bortezomib- and cisplatin-treated MCF7, HCC38, MCF-10A, and MDA-MB-436 breast cancer cells generated in the present study and Hafner et al.31 Error bars depict the standard error of the mean. T-test was used to calculate statistical significance (Benjamini-Hochberg adjusted p-values). ns = not significant (P > 0.05); *P ≤ 0.05; **P ≤ 0.01; ***P ≤ 0.001; ****P ≤ 0.0001. The dose-response curves and bar plots were generated using ggplot2 (version 3.2.1) in R51.
