Figure 1 | Scientific Reports

Figure 1

From: Acclimation of bacterial cell state for high-throughput enzyme engineering using a DmpR-dependent transcriptional activation system

Figure 1

Schematic depiction of the GESS and involved transcriptional factors in the host. Under stress conditions, (p)ppGpp can be synthesized by RelA/SpoT, which controls the expression levels of several transcription factors. Along with the target enzyme, the GESS consists of sigma 70 (σ70, rpoD)-dependent DmpR/enzyme expression systems and a σ54-dependent reporter expression system, including RNA polymerase (subunit α, β, β′ and ω corresponds to rpoA, rpoB, rpoC and rpoZ, respectively), SpoT (bifunctional (p)ppGpp synthase/hydrolase), RelA ((p)ppGpp synthase), σ54 (sigma 54 factor, rpoN) and IHF (heterodimer subunits, ihfA and ihfB).

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