Table 1 Systematic comparison of QuanTI-FRET method with previous work from Lee et al.25 and Chen et al.29 Dataset [A] was analyzed with the three methods, the resulting correction factors and FRET probabilities for C5V, C17V and C32V are given in this table, with the uncertainty on \({\beta }^{X}\) and \({\gamma }^{M}\) resulting from a different bootstrap analysis.
From: QuanTI-FRET: a framework for quantitative FRET measurements in living cells
\({{\boldsymbol{\beta }}}^{{\boldsymbol{X}}}\) | \({{\boldsymbol{\gamma }}}^{{\boldsymbol{M}}}\) | C5V | C17V | C32V | |
|---|---|---|---|---|---|
QuanTI-FRET | \(1.167\pm 0.008\) | \(2.10\pm 0.02\) | \(50.3\pm 0.4\) | \(41.7\pm 0.8\) | \(35.1\pm 0.8\) |
Lee et al.25 | \(1.13\pm 0.01\) | \(2.37\pm 0.05\) | \(47.5\pm 0.4\) | \(38.9\pm 0.8\) | \(32.4\pm 0.8\) |
Chen at al.29 | \(1.135\pm 0.005\) | \(2.19\pm 0.02\) | \(49.4\pm 0.4\) | \(40.8\pm 0.8\) | \(34.2\pm 0.8\) |
