Figure 4
The relationship between total dysfunction of CD8 and CD4 T cells and tumour grade. (a) Comparison of the expression ratio of each fraction of CD8 T cells stratified by tumour grade. (b) Comparison of the expression ratio of each fraction of CD4 T cells stratified by tumour grade. For this panel, the comparison was performed by Mann-Whitney U test. The central tendency of the box plot shows the median of each group, and the upper and lower ranges of the box plot show the 25th and 75th percentiles of each data set, respectively. *P < 0.05. (c) The ratio of the patients stratified by expression pattern of CD4 T cells and CD8 T cells with dysfunctional populations according to tumour grade. Dysfunctional populations were divided into two groups based on the median expression level (Fr. IV of CD8 T cells: 20.3%, Fr. V of CD4 T cells: 2.79%). (d) The ratio of the patients with high expression ratio of both CD4 T cells and CD8 T cells with dysfunctional populations according to tumour grade. ***P < 0.001. (e) Comparison of multiple cytokine productivity (IFNγ, TNFα, and IL-2) of CD8 TILs from 18 patients stratified by tumour grade within Fr. II, Fr. III, and Fr. V. (f) Comparison of multiple cytokine productivity (IFNγ, TNFα, and IL-2) of CD4 TILs from 18 patients stratified by tumour grade within Fr. II, Fr. III, and Fr. IV. **P < 0.01, *P < 0.05. (g) Cytotoxic functionality producing GZMB and both GZMB and PRF1 in Fr. II of CD8 T cells is compared according to tumour grade. Comparison of two sample types was performed by Mann-Whitney U test. The central tendency of the box plot indicates the median of each group, and the upper and lower ranges of the box plot show the 25th and 75th percentiles of each data set, respectively. *P < 0.05.
