Figure 1 | Scientific Reports

Figure 1

From: Differential kinase activity of ACVR1 G328V and R206H mutations with implications to possible TβRI cross-talk in diffuse intrinsic pontine glioma

Figure 1

The intrinsic ATPase activity wild-type, R206H and G328V mutants of ACVR1 kinase domains with 0.5 mM ATP in pH 7.3 kinase buffer. (a) 22 oC and (b) 37 oC. The turnover rate kobs is quantified based on the ADP produced and normalized by the native ACVR1 concentration (see Methods). *p < 0.05, **p < 0.01, ***p < 0.001, unpaired t-test. (c) Coomassie stained SDS-PAGE gel of purified ACVR1 kinase domain stocks used for kinase assays. See Fig. S5 for the full-length gel image.

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