Figure 4

Schematic of experimental procedure to analyze protective functions of antioxidants on particulate matter mucotoxicity. X. laevis embryos were pretreated with the indicated antioxidants trolox, NAC (N-acetyl cysteine), or α-tocopherol. After 1 h incubation in the antioxidant-containing media, embryos were transferred to PM treatment media. The level of secreted mucus was measured by ELLA assay using WGA-HRP. PM, particulate matter; ELLA, enzyme-linked lectin assay.