Figure 2

Malignant phenotypes of the B4GalNAc-T2 gene transfectant cells. (A) Effects of GalNAc-DSLc4 expression on the cell proliferation. Two transfectants and two vector controls (2.5 × 10³ cells/well) were seeded in 48-well plates in serum-containing medium and cultured for 6 days. The absorbance (590 nm) was measured on day 1, 2, 5, and 6. Data are means of three independent experiments. (B) Invasion activity of two transfectants and two vector controls. Cell invasion was analyzed by Boyden chamber invasion assay by counting the cell number on the reverse side of the filter. Data are means of three independent experiments. Bars indicate mean ± S.D. (n = 3). *, P < 0.05, **, P < 0.01,***, P < 0.005. (C) Dynamic monitoring of cell adhesion to LN, CL type I, CL type IV, or FN-coated surfaces. GalNAc-DSLc4-expressing cells and control cells were seeded in the wells of 96-well e-plate at 2.5 × 10⁴ cells/well with FCS, and cell attachment and spreading were monitored by RT-CES system. The e-plates were pre-coated with LN (a, b, c), CL type I (d), CL type IV (e), or FN (f). VMRC-RCZ transfectants were used in a and b, and THUR14TKB transfectants were used in c-f. Red and yellow lines mean transfectant cells, and green and blue lines mean control cells.