Figure 2 | Scientific Reports

Figure 2

From: Cell surface processing of the P1 adhesin of Mycoplasma pneumoniae identifies novel domains that bind host molecules

Figure 2

Immunoblots of cell lysates of M. pneumoniae probed with sera raised against regions within P1. Sera raised against 15 different regions (‘RP’ boxes) of P1 were a gift from R. Dumke55. (Top panel) Simplified cleavage map depicting the P1 adhesin, cleavage sites, and the 15 regions of P1 that have been previously cloned and expressed as recombinant fragments in E. coli55. The mass of full length and smaller proteoforms of P1 as predicted by ProtParam65. (Bottom panel) Immunoblots depicting M. pneumoniae cell lysate probed with the panel of anti-recombinant P1 sera. All the immunoblot lanes are part of the same blot. The membrane was blocked and then sliced to separate lanes before incubating with the described P1 sera. (Bottom right) Immunoblots with the intensity adjusted to highlight low abundant bands. Proteins migrating with masses similar to P1 proteoforms identified by LC-MS/MS have been marked on the immunoblot.

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