Figure 2
From: Generation of intestinal organoids derived from human pluripotent stem cells for drug testing
Purification of hiPSC-IECs from intestinal organoids. (A) The immuno-staining images of EpCAM in intestinal organoids are shown. The scale bar represents 200 μm. (B) A schematic drawing of the assay procedure. (C) The proportion of EpCAM+ cells in pre-separation cells before MACS, positive fraction after MACS and negative fraction after MACS are analyzed by flow cytometry. (D) Representative immuno-staining images of VILLIN, LYZ, MUC2, CHGA, and EphB2 in EpCAM+ cells (from the left), and bright field image (the rightmost panel) seeded on a culture plate. A scale bar represents 200 µm. (E) Intestinal stem cell marker (LGR5) and transient amplifying cell maker (EPHB2) gene expressions in EpCAM+ cells. Data are presented as means ± S.D. (n = 3).
