Table 2 Thermal cycling conditions for qPCR analysis.

From: Quantitative Analysis of Salivary Oral Bacteria Associated with Severe Early Childhood Caries and Construction of Caries Assessment Model

Species

Thermal cycling conditions

Pre-heating

denaturation

annealing

elongation

cycles

Prevotella denticola

94 °C, 10 min

94 °C, 15 s

60 °C, 20 s

72 °C, 15 s

30

Prevotella pallens

94 °C, 10 min

94 °C, 15 s

60 °C, 20 s

72 °C, 15 s

30

Streptococcus mutans

94 °C, 10 min

95 °C, 10 s

62–72 °C, 30 s

70 °C, 30 s

30

Lactobacillus fermentum

95 °C, 10 min

95 °C, 15 s

62 °C, 1 min

50 °C, 2 min

30

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