Figure 6
Effects of Giardia trophozoites infection of human macrophages on the levels of NF-κB p65 subunit. Cells (6 × 105) were maintained in culture medium (control), or pre-incubated with G. lamblia (3 × 106 cells) for 1 h, and then activated with 1 μg/ml LPS for 30 minutes. Total cell extracts were analyzed by Western blot using antibodies against NF-κB p65. An anti-tubulin antibody was used to confirm equal protein loading. Results were expressed as percentage of NF-κB p65 protein levels relatively to LPS. Each value represents the mean ± SEM from at least 3 independent experiments (***p < 0.001, compared to control; ####p < 0.0001, compared to LPS).
