Figure 2
Effect of fasentin on endothelial cell cycle distribution. (a) HMECs were exposed for 24 h to fasentin at the indicated concentrations, stained with propidium iodide and percentages of cells on subG1, G1, S and G2/M phases were determined using a FACS VERSETM cytometer. (b) 10 μM EdU was added for 2 h to cells treated with fasentin for 22 h and EdU incorporation was determined using a FACS VERSETM cytometer. A representative result and quantification, expressed as means ± SD of three independent experiments, are shown. *p < 0.05, **p < 0.01, ****p < 0.0001 versus untreated control.
