Figure 6
Fasentin decreases the levels of molecules related to the remodelling of the extracellular matrix and inhibits EC invasion in HMECs. (a) Cell extracts from HMECs treated for 16 h with the indicated concentrations of fasentin were normalized for equal cell density and used for gelatine zymography as indicated in the “Methods” section. (b) Quantification of the relative MMP-2 levels. (c) Cells extracts from the control condition were used for in situ gelatine zymography. 100 μM fasentin was added to cut lanes of the gel, incubated for 16 h and stained with Coomassie Brilliant Blue. (d) Representative image of uPA activity in cell lysates from control and fasentin-treated HMECs for 16 h. (e) Quantification of the relative uPA levels. (f) Relative MMP-2 mRNA expression after 8 h treatment with 100 μM fasentin. (g) Representative photographs of control and fasentin-treated HMECs after 16 h incubation in a transwell insert coated with Matrigel. FBS was added as chemoattractant to the lower wells (bar = 200 μm). (h) Quantification of invasive cells. Data are given as percentage of the untreated control, and expressed as means ± SD of three independent experiments. *p < 0.05, **p < 0.01, ***p < 0.001 versus untreated control. Uncropped blots are shown in the supplementary information.
