Table 1 Comparison of plasma qPCR and skin snip parasitological evaluation.

From: The insufficiency of circulating miRNA and DNA as diagnostic tools or as biomarkers of treatment efficacy for Onchocerca volvulus

 

Baseline

Month four

Month 12

Month 21

Treatment

Patient

qPCR

mf/snip

qPCR

mf/snip

qPCR

mf/snip

qPCR

mf/snip

DOXY

a.1

Neg

40

Neg

1

Neg

2

Neg

Neg

a.2

39.26 ± 1.24

15.5

Neg

34

Neg

6.5

Neg

Neg

a.3

37.28 ± 1.05

14

Neg

2

Neg

Neg

Neg

Neg

a.4

38.22 ± 1.24

20.5

Neg

10

Neg

Neg

Neg

Neg

a.5

Neg

16.5

Neg

2

Neg

Neg

Neg

3.5

a.6

Neg

23.5

Neg

1

Neg

Neg

Neg

8.5

a.7

34.31 ± 0.54

13

36.88 ± 0.44

Neg

38.43 ± 1.02

1

37.47 ± 0.65

Neg

a.8

Neg

19

Neg

93

Neg

11.5

Neg

Neg

a.9

Neg

45

Neg

6

Neg

Neg

Neg

Neg

DOXY + IVM

b.1

Neg

18

Neg

9

Neg

1.5

Neg

Neg

b.2

Neg

24

Neg

1

Neg

Neg

Neg

Neg

b.3

38.79 ± 0.52

34.5

Neg

13

Neg

0.5

Neg

Neg

b.4

38.37 ± 1.66

88

38.61 ± 0.84

Neg

Neg

Neg

Neg

1

b.5

38.61 ± 0.46

61.5

Neg

28

Neg

Neg

Neg

Neg

IVM

c.1

38.26 ± 0.26

90.5

36.03 ± 0.38

99

Neg

0.5

Neg

Neg

c.2

Neg

12

Neg

Neg

Neg

Neg

Neg

0.5

c.3

Neg

15.5

Neg

1

Neg

Neg

Neg

2

c.4

Neg

23.5

38.42 ± 0.99

2

Neg

30

Neg

8.5

  1. Trial participants screened longitudinally for circulating O-150 DNA by qPCR and their corresponding average mf/snip (mf/mg) are detailed. Mean Cq value and ± SD for positive results are displayed. Cq, quantification cycle; mf, microfilariae; Neg, negative; SD, standard deviation.