Figure 11

Histopathological structures of skin layers showing changes in the normal structures after intake of HFCS and MNs loaded with V.agnus-castus and T.indica (200 mg/kg). (A) Normal control group with no histopathological alteration and normal epidermis with stratified keratinized epithelium and the underlying areolar connective tissue dermis with hair follicles and glands and lastly the skeletal muscle layer. (B) HFCS group: Focal acanthosis was detected in the prickle cell layer of the epidermis (→). The underlying dermis showed oedema, inflammatory cells infiltration and fibroblastic cells proliferation, which were extended deep with appearance of eosinophils infiltration. Necrobiosis was detected in the epithelial cells of some hair follicles. (C,D) V. agnus-castus group: There were hyperkeratosis of the epidermal layer associated with focal hyalinosis in the areolar tissue of the dermis. (E,F) T. indica group: The epidermal and dermal layers were histological intact but there was necrobiosis in some individual hair follicles.