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Figure 1

From: Cardiolipin is required in vivo for the stability of bacterial translocon and optimal membrane protein translocation and insertion

Figure 1

The ∆clsABC1 E. coli BKT12 mutant lacks detectible CL regardless of growth phase. The cells were grown in the presence of [32P]PO4. Strain W3110 (WT) and BKT12 ∆clsABC1 strains with the indicated cls gene deletions were grown in LB medium containing 10 g/L of NaCl and [32P]PO4 to either mid-log phase (log) or to stationary phase (stat). Phospholipids were extracted and analyzed by one-dimensional TLC as described in Materials and Methods.

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