Figure 3 | Scientific Reports

Figure 3

From: Fluid shear stress coupled with narrow constrictions induce cell type-dependent morphological and molecular changes in SK-BR-3 and MDA-MB-231 cells

Figure 3

Morphological analysis of SK-BR-3 and MDA-MB-231 cells post-circulation, -confinement and –constrictions at a constant applied pressure of 10 kPa. (a) DAPI staining of the nucleus. Representative images of the mean values of nucleus area have been chosen for SK-BR-3 (top row) and MDA-MB-231 (bottom row) cells for each condition. (b) DAPI staining of the nucleus. Representative images of the mean values of circularity have been chosen for SK-BR-3 (top row) and MDA-MB-231 (bottom row) nuclei for each condition. (c) Box plot showing all points (minimum to maximum) of the nucleus area for both cell types. (d) Box plot showing all points (minimum to maximum) of the cell area for both cell types. (e-g) Bar charts of the N:C ratio, circularity of the nucleus and aspect ratio of the nucleus for both cell types respectively. Error bars represent standard error of mean. Kolmogorov-Smirnov test. *significance level at 0.033; **significance level at 0.002; ***significance level < 0.001; ns, not significant.

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