Figure 4 | Scientific Reports

Figure 4

From: RAC1-Dependent ORAI1 Translocation to the Leading Edge Supports Lamellipodia Formation and Directional Persistence

Figure 4

RAC1 regulated the dynamics of peripheral ORAI1-CTTN and ruffling. U2OS cells stably expressing Flag-RAC1 (wild-type), Flag-RAC1G12V or Flag-RAC1T17N, were transfected for the transient expression of ORAI1-GFP and mCherry-CTTN. Fluorescence was monitored in live cells in Leibovitz’s L-15 medium supplemented with 10% FBS. The ROI indicated by the arrow was selected to assess the variation of fluorescence intensity (right panels). Emission of fluorescence of ORAI1-GFP (green line) and mCherry-CTTN (red line) was recorded every 3 sec for 4 min. Full time-lapse sequences are shown as Supplementary Movies S8 (for wild-type RAC1), S9 (RAC1G12V), S10 (RAC1T17N). Bar = 10 μm.

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