Figure 3

C-MYB activates Wnt signaling through miR-21. Wnt signaling was assessed through phosphorylation of β-catenin, as detected by ELISA, and endpoint measurement of absorbance at 450 nm. ES2 (A) and OVCAR3 (B) were transfected with c-MYB alone or with c-MYB and anti-miR-21, and then β-catenin was detected by ELISA. *p < 0.05 and #p < 0.01, compared to control.