Figure 3

Tomatidine reduces CHIKV infection when added up to 6 hpi. (a) Outline of the experimental set-up. (b) Huh7 cells were infected with CHIKV-LR at MOI 1 and treated with 10 µM tomatidine or the equivalent volume of EtOH prior to infection (−2 or −1 h), during infection or at different times post-infection (2 to 8 hpi). Supernatants were collected 9 hpi. Data is represented as mean ± SEM from four independent experiments and differences were assessed with Student’s t-test.