Figure 2 | Scientific Reports

Figure 2

From: CREB1 is affected by the microRNAs miR-22-3p, miR-26a-5p, miR-27a-3p, and miR-221-3p and correlates with adverse clinicopathological features in renal cell carcinoma

Figure 2

ccRCC cell line MZ2733RC as a model for the identification of unknown CREB1-specific post-transcriptional regulators. (A) Western blot-based detection and densitometric quantification of CREB1 protein in the ccRCC cell line MZ2733RC and the corresponding normal tissue cell line MZ2733NN. Number in parentheses indicates a technical replicate. Same blot was re-probed for β-Actin which served as loading control. Uncropped blot is shown in Supplementary Figure 3. (B) CREB1 mRNA levels analyzed by qRT-PCR in the indicated cell lines. GAPDH was used for normalization. (C) Expression levels of validated CREB1-specific miRNAs given as fold enrichment for MZ2733RC (RCC) cell line relative to MZ2733NN (NN) cell line analyzed by RNA-sequencing and qRT-PCR. Values above 1 (red line) indicate miRNAs enriched in the tumor cell line. n.d. not detectable.

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