Figure 3

Leukocyte infiltrates are similar across the mouse groups. Panel (a) shows hematoxylin and eosin staining of ear skin tissue; first column represents whole tissue appearance, while the second and third columns show the dermal and epidermal compartment; fourth column shows representative pictures of mouse ears immunostained with CD45 (leukocyte common antigen) antibody. Arrows indicate mononuclear leukocyte and granulocyte infiltrates in the dermis and epidermis. Original magnification x400. Scale bar 50 μm. Panels (b,c) represent quantification of mononuclear leukocytes and granulocytes (b), and CD45+ cells (c) in the dermal and epidermal compartments (GraphPad Prism v7.0). Data are represented as median value.