Table 2 Primary and secondary antibodies used for immunohistochemistry.

From: α-synuclein inclusions are abundant in non-neuronal cells in the anterior olfactory nucleus of the Parkinson’s disease olfactory bulb

Primary Antibodies

Antibody

Species

Dilution

Catalogue #

Manufacturer

α-synuclein (phospho s129)

Mouse

1:3,000

ab184674

Abcam

α-synuclein (phospho s129)

Rabbit

1:4,000

ab190628

Abcam

PDGFR-β [Y92]

Rabbit

1:200

ab32570

Abcam

GFAP

Chicken

1:4,000

ab4674

Abcam

Iba-1

Chicken

1:250

ab139590

Abcam

NeuN

Chicken

1:500

MABN91

EMD Millipore

PGP9.5

Mouse

1:1,000

ab8189

Abcam

UEA-1

1:500

DL-1067

Vector Laboratories

CNPase

Mouse

1:500

sc166558

Santa Cruz Biotechnology

Secondary Antibodies

Antibody

Dilution

Catalogue #

Manufacturer

 

Goat anti – mouse (488)

1:400

A11029

Thermofisher Scientific

 

Goat anti – rabbit (594)

1:400

A11037

Thermofisher Scientific

 

Goat anti – chicken (647)

1:400

A21449

Thermofisher Scientific

 

Strepavidin – (647)

1:500

S21374

Thermofisher Scientific