Table 2 Primary and secondary antibodies used for immunohistochemistry.
Primary Antibodies | ||||
|---|---|---|---|---|
Antibody | Species | Dilution | Catalogue # | Manufacturer |
α-synuclein (phospho s129) | Mouse | 1:3,000 | ab184674 | Abcam |
α-synuclein (phospho s129) | Rabbit | 1:4,000 | ab190628 | Abcam |
PDGFR-β [Y92] | Rabbit | 1:200 | ab32570 | Abcam |
GFAP | Chicken | 1:4,000 | ab4674 | Abcam |
Iba-1 | Chicken | 1:250 | ab139590 | Abcam |
NeuN | Chicken | 1:500 | MABN91 | EMD Millipore |
PGP9.5 | Mouse | 1:1,000 | ab8189 | Abcam |
UEA-1 | — | 1:500 | DL-1067 | Vector Laboratories |
CNPase | Mouse | 1:500 | sc166558 | Santa Cruz Biotechnology |
Secondary Antibodies | ||||
Antibody | Dilution | Catalogue # | Manufacturer | |
Goat anti – mouse (488) | 1:400 | A11029 | Thermofisher Scientific | |
Goat anti – rabbit (594) | 1:400 | A11037 | Thermofisher Scientific | |
Goat anti – chicken (647) | 1:400 | A21449 | Thermofisher Scientific | |
Strepavidin – (647) | 1:500 | S21374 | Thermofisher Scientific | |