Figure 3
Pyk2 is implicated in acute cocaine responses. (A), Immunoblot for phospho-tyrosine and total Pyk2 after Pyk2 immunoprecipitation (IP) from striatal extracts of wild-type mice killed 10 min after i.p. injection of saline (Sal) or cocaine (20 mg/kg, Coc). (B), Densitometry quantification of results as in A. Data are ratios of phospho/total Pyk2 for each sample and expressed as percentage of the mean ratios in saline-treated samples (n = 7 mice per group). (C), Locomotor activity of Pyk2+/+ (n = 10 mice) and Pyk2-/- mice (n = 9) after a first cocaine injection (20 mg/kg, Coc, arrow) 30 min after mice were placed in the open field. (D), The same mice received a second injection of cocaine in the same conditions as in C, 13 days later. (E), Pairwise comparison of the distance traveled 0–15 minutes following the first and the second injection in Pyk2+/+ and Pyk2−/− mice (from data in C and D). (F), Sensitization ratios calculated as the distance traveled 0–15 min after the second injection (day 14) divided by the distance traveled during the same period after the first (day 1) cocaine injection (from data in E), in Pyk2+/+ and Pyk2−/− mice. (B-F), Data are means ± SEM, indicated by a shaded area in C and D, and horizontal bars in B, E and F. t-test in B and F, 2-way ANOVA followed by post-hoc Sidak’s multiple comparisons test in C-E. ns, not significant, *p < 0.05, **p < 0.01. See Supplementary Table 1 for detailed statistical analyses and Supplementary Figure 3 for full length blots.
