Figure 4
Specific deletion of Pyk2 in the NAc but not in the DS alters the acute locomotor response to cocaine. (A), Pyk2f/f mice were bilaterally injected in the NAc with AAV expressing GFP (Pyk2f/f;NAc,GFP) or Cre and GFP (Pyk2f/f;NAc,GFP-Cre). A representative section through a Pyk2f/f;NAc,GFP-Cre striatum is shown (left panel, Pyk2 immunofluorescence, right panel, Pyk2, red, and GFP, green, double fluorescence). The location of the tips of injecting cannula in mice used for further analysis is summarized (right panel, schematic brain section from Paxinos and Franklin64). (B), Locomotor activity of the mice injected in the NAc after the first injection of cocaine (20 mg/kg, Coc, arrow), as in Fig. 3C (Pyk2f/f;NAc,GFP, n = 11 mice) or Pyk2f/f;NAc,GFP-Cre, n = 10 mice). (C), The same mice received a 2nd injection of cocaine, 13 days later. (D), Pairwise comparison of the distance traveled 0–15 minutes following the first and the second injection in Pyk2f/f;NAc,GFP and Pyk2f/f;NAc,GFP-Cre mice (from data in B and C). (E), Sensitization ratio as in Fig. 3F (distance traveled 0–15 min after the second injection [day 14, D] / distance traveled during the same period after the first injection [day 1, C]). (F-J), Same as in A-E, except that mice were injected into the DS, not the NAc. (F), Anatomical verification as in A. (G, H), Locomotor activity of Pyk2f/f;DS,GFP (n = 8 mice) and Pyk2f/f;DS,GFP-Cre mice (n = 7) after the first (G) and the second (H, 13 days later) injection of 20 mg/kg cocaine. I, Pairwise comparison of the distance traveled 0–15 minutes following the first and the second injection in Pyk2f/f;DS,GFP and Pyk2f/f;DS,GFP-Cre mice (from data in G and H). J, Sensitization ratios, as in E, in Pyk2f/f;DS,GFP and Pyk2f/f;DS,GFP-Cre mice. (B-E and G-J), Data are means ± SEM, indicated by a shaded area in B, C, G, and H, and horizontal bars in E and J. In B-D and G-I, data were analyzed with 2-way ANOVA followed by post-hoc Sidak’s multiple comparisons test, and in E and I by Mann and Whitney test. ns, not significant, *p < 0.05, **p < 0.01, ***p < 0.001. See Supplementary Table 1 for detailed statistical analyses.
