Figure 7
Pyk2 deletion does not alter cocaine-conditioned place preference. For each group of Pyk2 mutant mice and their respective matched controls, the time spent in the cocaine-paired arm before (Day 0) and after (Day 7) cocaine (15 mg/kg) conditioning was measured (see Materials and Methods). (A), Time spent in the cocaine-paired arm at days 0 and 7 by Pyk2+/+ (n = 10 mice) and Pyk2-/- mice (n = 9). (B), The CPP score was calculated for each Pyk2+/+ and Pyk2-/- mouse in A as the excess time spent in the cocaine-paired arm. (C), CPP score as in B for in mice injected in the NAc with AAV expressing GFP or Cre and GFP (Pyk2f/f;NAc,GFP, n = 18, and Pyk2f/f;NAc,GFP-Cre mice, n = 15). (D), Same as in C, except that the injection was in the DS (Pyk2f/f;DS,GFP, n = 8, and Pyk2f/f;DS,GPF-Cre, n = 7). (E), Same as in B, in Pyk2f/f (n = 13) and Pyk2f/f;D1::Cre (n = 12) mice. (F), Same as in B, in Pyk2f/f (n = 14) and Pyk2f/f;A2A::Cre (n = 12) mice. B-F Bars are means + SEM. Analysis was done with 2-way ANOVA in A followed by Sidak’s test, and with unpaired Mann-Whitney test in B-F (see Results). See Supplementary Table 1 for detailed statistical analyses.
