Figure 4
From: Melatonin modulates red-ox state and decreases viability of rat pancreatic stellate cells
Effect of melatonin on mitochondrial membrane potential. PSC were loaded with the mitochondria-specific voltage-sensitive dye TMRM. The cells were then incubated during 1 h in the presence of melatonin (1 mM, 100 µM, 10 µM or 1 µM). As a control, different batches of cells were incubated in the presence of the mitochondrial uncoupler CCCP (100 nM). The bars show the changes in ψm of treated and non-stimulated (control) cells, and are presented as the mean increase of fluorescence expressed in percentage ± SEM with respect to non-stimulated cells. A horizontal dotted line represents the value observed in non-stimulated cells (n.e., non-stimulated cells; Mel, melatonin; ***P < 0.001 vs non-stimulated cells; n = four independent experiments).
