Figure 5
From: Melatonin modulates red-ox state and decreases viability of rat pancreatic stellate cells
Effect of melatonin on glutathione. (A) PSC were incubated during four h in the presence of melatonin (1 mM, 100 µM, 10 µM or 1 µM), and the effect on glutathione was analyzed. The bars show the mean increase in GSH/GSSG ratio expressed in percentage ± SEM with respect to non-stimulated cells. (B-D) RT-qPCR analysis of Nrf2-target genes glutamate cysteine ligase-catalytic subunit (GClc), catalase (CAT), NAD(P)H quinone oxidoreductase 1 (NQO1) and heme-oxygenase-1 (HO-1) reveals statistically significant increases in the levels of Nrf2-dependent antioxidant enzymes in cells incubated in the presence of melatonin. Incubation of cells with H2O2 (100 µM) also evoked an increase in the expression of all four antioxidant enzymes. Gapdh mRNA was used for normalization. Data are expressed as the mean ± S.E.M. of the change relative to non-stimulated cells. A horizontal dotted line represents the value observed in non-stimulated cells. Three different cellular preparations were used (n.e., non-stimulated cells; Mel, melatonin; *P < 0.05; **P < 0.01).
