Figure 7
HSV-1 decreased phosphorylated MEK1/2, and MG132 increased cytoplasmic phosphorylated MEK1/2 in virus infected cells. Subcellular localization of the phospho-MEK1/2 in HSV-infected and MG132-treated cells. Vero cells were infected with HSV-1 at an MOI of 1 and treated with (or without) 0.75 μM MG132 for 10 hours. Vero cells were stained with anti-Ser217/Ser221-phospho-MEK1/2 (green), anti-ICP5 (red) and DAPI (blue) and observed by laser scanning confocal microscopy with a 40x water-immersion objective. Scale bars represent 20 μm. The upper and middle images show gray-scale images (single channel) of phospho-MEK1/2 and ICP5, respectively, and the lower images show triple staining of phospho-MEK1/2, ICP5 and DAPI.
