Table 3 Primers used in this study.
Name | Sequences (5′-3′) | Annealing Temp | Extension time | Cycle no. |
|---|---|---|---|---|
PCR (gDNA amplification) | ||||
intron 1F exon 2R | cagtctagacagcagggcaa agtaggatgtgccccaggag | 55 °C | 1 min | 35 |
exon 16F exon 20R | caaggactctagcacctgg gaatctcccaagtcctgtga | 60 °C | 3 min | 34 |
intron 14F exon 15R | catgctgggtggctgagaa ctcctcgtgtgtactacggc | 60 °C | 1 min | 28 |
intron 5F intron 8R | cagagccctccaagtgaaga ttccaggaccaggtgacatc | 60 °C | 1 min | 30 |
exon 10F intron 12R | actgccttccccgacttca ggagctttctgggatgag | 60 °C | 5 min | 34 |
RT-PCR (GAA transcript amplification) | ||||
exon 1F exon 5R | ggaaactgaggcacggagcg ggaccacatccatggcattgc | 60 °C | 2 min | 34 |
exon 4F exon 10R | gtatatcacaggcctcgccg ctggtcatggaactcagcca | 60 °C | 2 min | 34 |
exon 9F exon 17R | gggggttttcatcaccaacga ctgccaagggcctctactgg | 60 °C | 2 min | 34 |
exon 16F exon 20R | caaggactctagcacctgg gaatctcccaagtcctgtga | 60 °C | 2 min | 34 |
exon 4F exon 19R | gtatatcacaggcctcgccg gaagttggagacagggacacc | 60 °C | 3 min | 35 |
qPCR | ||||
GAA transcript (exon 1-2), amplification exon 1F(q) exon 1-2R(q) | tgggaaagctgaggttgtcg tcctacaggcccgctcc | Refer to Methods | ||
TBP transcript (exon 1-2), amplification exon 1-2F(q) exon 2R(q) | tctttgcagtgacccagcatcac cctagagcatctccagcacactct | |||
