Figure 5
RNA-Seq data analysis of TRAP-extracted polysomal RNA from MacTRAP kidneys. (A) Principle component analysis (PCA) shows distinct clustering between unbound (blue dots) and bound (red dots) fractions extracted from kidneys of 9-week- old MacTRAP mice. (B) Heatmap of RNA-Seq data illustrating the 100 most differentially expressed genes between bound (renal macrophage-specific) vs. unbound (whole kidney) fractions. (C) MA plot of RNA-Seq data shows gene expression in relation to mean normalized counts. Macrophage-specific transcripts are robustly enriched in the bound vs. unbound fraction (selected genes are highlighted, red font), whereas transcripts representative of other cell types are depleted (blue font); red dots represent differentially expressed genes with p adj. < 0.05.
