Figure 1
From: NusA directly interacts with antitermination factor Q from phage λ
Structure of λQΔ36. (a) Ribbon representation of the crystal structure of a λQ truncation variant lacking 38 residues at the N-terminus, electron density was only interpretable starting at residue 62 (PDB ID: 4MO1; gray). Termini and secondary structure elements are labeled. Unassigned amide backbone signals are shown in black, the Zn2+ is highlighted as cyan sphere. The PyMOL Molecular Graphics System (Version 1.7, Schrödinger, LLC.; https://pymol.org) was used for visualization. (b) Overlay of [1H,15N]-BEST-TROSY spectra of 15N-λQ (red) and 15N-λQΔ36 (black). (c) Chemical shift index (CSI) for Cα and CO of λQΔ36 vs. secondary structure of the crystal structure (PDB ID: 4MO1). The missing residues due to non-interpretable electron density are indicated in red, the loop region is shown in cyan.
