Figure 4

Analysis of motility and autoagglutination activity. (A) Migration (mm) of bacteria from the inoculation site for WT, ΔcheV and ΔcheW isogenic mutants in triplicate; (B–D) Images of the formed halos demonstrating decreased swarming motility by WT, ΔcheV and ΔcheW mutants; (E) Spectrophotomer readings of the upper phase of cell suspension following liquid culture demonstrate a lower absorbance in ΔcheV and ΔcheW strains when compared to WT, indicating an increased tendency for cells to autoagglutinate, (F–H) which was also evident when culture tubes were inspected.