Figure 2

There was no changes on the [Ca²⁺]_i concentration in the HEK293 cells (in the absence of TRPM2) by hydrogen peroxide (H₂O₂). (mean ± SD). The cells were stained with Fluo-8 calcium dye and mean ± SD of fluorescence in 15 mm² of the cells as arbitrary unit are presented; n = 20 independent experiments. The HEK293 cells were stimulated by H₂O₂ (1 mM for 10 min), but they were inhibited by 2-APB (100 μM for 10 min). The samples were analyzed by the LSC microscopy fitted with a 40× oil objective. The scale bar was 5 µm. Representative images (a) and column (b) of fluorescence intensities of the H₂O₂ and 2-APB on the TRPM2 activation in the LSC microscope analyses are shown in Figs a and b, respectively.