Figure 3
From: Hepatocyte-specific deletion of Pparα promotes NAFLD in the context of obesity
Analysis of the liver transcriptome in WT, Pparαhep−/−, and Pparα−/− mice in response to HFD. WT, Pparαhep−/−, and Pparα−/− mice were fed a control diet (CTRL) or a HFD for 10 weeks at 30 °C (thermoneutrality). A transcriptomic analysis performed with liver samples from WT, Pparαhep−/−, and Pparα−/− exposed or not exposed to HFD (n = 8 mice/group) revealed 7173 differentially regulated genes (FDR < 5%). (a) Heat map of microarray expression data from 7173 regulated genes. Red and green indicate values above and below the mean averaged centred and scaled expression values (Z-score), respectively. Black indicates values close to the mean. According to the probe clustering (left panel), 12 gene clusters exhibited specific gene expression profiles. (b) sPLS-DA representing the 7173 differentially expressed genes from the microarray. (c) Correlation analysis between either WT, Pparαhep−/−, and Pparα−/− mice for the effect of HFD. (d) Relative hepatic expression of Vnn1, Fmo3, Col1a1 and Pparγ2 quantified by qPCR. Data represent mean ± SEM. #, significant diet effect and *, significant genotype effect. # or *p ≤ 0.05; ## or **p ≤ 0.01; ### or ***p ≤ 0.001. (e) Venn diagrams comparing the number of genes significantly regulated under HFD in the livers of WT, Pparαhep−/−, and Pparα−/− mice at adjusted p-value < 0.05.
