Figure 1 | Scientific Reports

Figure 1

From: Transcriptional analyses of differential cultivars during resistant and susceptible interactions with Peronospora effusa, the causal agent of spinach downy mildew

Figure 1

Experimental design and sample collection for RNA sequencing analyses of resistant and susceptible spinach cultivars in response to the spinach downy mildew pathogen, Peronospora effusa. Two-week-old spinach seedlings of the differential cultivars were inoculated with P. effusa sporangial suspensions. Leaves of the downy mildew resistant cultivar Solomon and susceptible cultivar Viroflay were collected at 48 and 168 hpi, and immediately transferred in liquid nitrogen. Un-inoculated control seedlings were also incubated overnight in the dew chamber, prior to collecting leaf samples. Three leaves per plant per treatment were pooled for total RNA extraction. cDNA libraries were prepared using three separate biological replicates per treatment.

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