Figure 6

Cell viability (a) and DNA synthesis (b,c) quantified in STIM1/2- deficient primary murine lung fibroblasts (pmLF) compared to control cells. (a) Viability was analyzed in wild type pmLF infected with recombinant lentiviruses expressing Cre recombinase (Wt Cre) as well as STIM1/2- deficient pmLF using a WST-assay 5–8 days after infection. (b) Wild type pmLF infected with recombinant lentiviruses expressing Cre recombinase (Wt Cre) as well as STIM1/2- deficient pmLF were incubated with EdU (5-ethynyl-2′-deoxyuridine) for 4 hours and fixed cells were stained with cross- linked fluorescent azide. Nuclei staining was performed by Hoechst dye. (c) Individual values and means +/− SEM of EdU/Hoechst ratios were plotted . Asterisks mark significant differences (n > 3 mice, **P < 0.01) between ratios of STIM1/2-deficient cells compared to control cells.