Figure 7

Quantification of nuclear NFATc levels in STIM1/2- deficient pmLF (STIM1/2^ΔpmLF) and wild-type cells infected with recombinant lentiviruses expressing Cre recombinase as control cells (Wt Cre). Representative Western Blots showing NFATc1 (left panel) and NFATc3 (right panel) isoforms in nuclear extracts from STIM1/2^ΔpmLF and Wt Cre cells. Summary of quantitative analysis of nuclear NFAT levels of the c1 (upper bar graph) and c3 (lower bar graph) isoforms. Columns show calculated means +/− SEM. Asterisks mark significant differences (n > 3 mice, *P < 0.05, **P < 0.001) between ratios of STIM1/2^ΔpmLF cells compared to Wt Cre control cells.