Table 1 Primer pairs used for amplification of quantitative RT-PCR fragments.
Target | Species | Forward primer (5′-3′) | Reverse primer (5′-3′) |
|---|---|---|---|
STIM1 | mouse | AAG CTT ATC AGC GTG GAG GA | CCT CAT CCA CAG TCC AGT TGT |
STIM2 | mouse | GAG GGC GCA GAG TGT GAG | TTT AGA GCC ATG CGG ACC T |
Orai1 | mouse | TAC TTA AGC CGC GCC AAG | ACT TCC ACC ATC GCT ACC A |
Orai2 | mouse | GGA CCT CAG CCC TCC TGT | GGG TAC TGG TAC TTG GTC TCC A |
Orai3 | mouse | CAC ATC TGC TCT GCT GTC G | GGT GGG TAT TCA TGA TCG TTC T |
TRPC1 | mouse | TGA ACT TAG TGC TGA CTT AAA GGA AC | CGG GCT AGC TCT TCA TAA TCA |
TRPC3 | mouse | TGG ATT GCA CCT TGT AGC AG | ACC CAG AAA GAT GAT GAA GGA G |
TRPC4 | mouse | GAT GAT ATT ACC GTG GGT CCT G | GAT TCC ACC AGT CAT GGA TGT |
TRPC5 | mouse | CTC TAC GCC ATC CGC AAG | TCA TCA GCG TGG GAA CCT |
TRPC6 | mouse | GCA GCT GTT CAG GAT GAA AAC | TTC AGC CCA TAT CAT GCC TA |
TRPC7 | mouse | AAT GGC GAT GTG AAC TTG C | CAG TTA GGG TGA GCA ACG AAC |
β-actin | mouse | CTA AGG CCA ACC GTG AAA AG | ACC AGA GGC ATA CAG GGA CA |
