Figure 2
From: Smooth-muscle-derived WNT5A augments allergen-induced airway remodelling and Th2 type inflammation
Airway remodelling in OVA-treated TetO-Wnt5a;SM22-rtTA mice. (A) Time overview of the ovalbumin (OVA) protocol. Mice were initially sensitised three times to OVA and subsequently challenged with saline or OVA for four weeks. Doxycycline (DOX) was administered through the drinking water. (B) WNT5A immunoblot of whole lung wild type homogenates (treated as in (A)), normalised against β-actin. (C) Representative immunohistochemistry image (left) and the quantification (right) of an alpha smooth muscle actin (α-SMA) staining of wild type (WT) and transgenic (Tg) mouse airways exposed to repeated allergen (ovalbumin; OVA) challenge vs PBS-treated controls. (D) Histological staining as in (C) of a periodic acid Schiff staining, indicative of mucus. (E) MUC5A relative gene expression of whole lung homogenates subjected to RT-qPCR. (F) Collagen 1α1 immunoblot of whole lung homogenates (treated as in (A)), normalised against β-actin. One-way ANOVA followed by Tukey’s post hoc test. Group sizes are n = 6, 8, 5, 6 for the respective groups (left to right). Data are expressed as the mean ± SEM. *p < 0.05, **p < 0.01, ***p < 0.001.
