Table 1 Primer sequences.
From: Smooth-muscle-derived WNT5A augments allergen-induced airway remodelling and Th2 type inflammation
Amplicon | Species | Forward sequence (5′→3′) | Reverse sequence (5′→3′) |
|---|---|---|---|
rtTA | Mus Musculus | CGCTGTGGGGCATTTTACTTTAG | CATGTCCAGATCGAAATCGTC |
TetO-Wnt5a | Mus Musculus | TCGTACCTAGAGACCACCAAG | CTACACCCTGGTCATCATCC |
MUC5A | Mus Musculus | GAGATGGAGGATCTGGGTCA | GCAGAAGCAGGGAGTGGTAG |
IL4 | Mus Musculus | TGACGGCACAGAGCTATTGA | TTTGGCACATCCATCTCCGT |
IL5 | Mus Musculus | GGGGGTACTGTGGAAATGCT | AATCCAGGAACTGCCTCGTC |
IL13 | Mus Musculus | AAACTGCAGCAAGACCGTGA | CCACCGGGATACTGACAGAC |
18S ribosomal RNA | Mus Musculus | AAACGGCTACCACATCCAAG | CCTCCAATGGATCCTCGTTA |
B2M | Mus Musculus | ACCGTCTACTGGGATCGAGA | TGCTATTTCTTTCTGCGTGCA |
RPL13A | Mus Musculus | AGAAGCAGATCTTGAGGTTACGG | GTTCACACCAGGAGTCCGTT |
IL31 | Mus Musculus | AACAACGAAGCCTACCCTGG | GGTTAATGCTTCCCGGTCCA |
18 S | Homo Sapiens | GGATGCGTGCATTTATCAGA | GTTTCTCAGGCTCCCTCTCC |
IL31 | Homo Sapiens | CCTCGACGTCTGTGCTCTTT | TTGAGATATGCCCGGATGGC |
