Figure 2
From: Chronic Intermittent Hypoxia Triggers a Senescence-like Phenotype in Human White Preadipocytes
P16 and γH2AX expression is increased with chronic exposure to intermittent hypoxia (IH). Representative Western bolts and graphs showing upregulation of p16 (a) and γH2AX (b) protein expression with IH treatment. Representative confocal images showing increased nuclear localization of p16 (green) and γH2AX (red) in cells exposed to IH (c). Nuclei are counterstained blue (DAPI). White arrows indicate positive nuclei. Quantitation of cells positive for nuclear p16 (d), γH2AX (e) and p16&γH2AX (f) in preadipocytes grown in continuous normoxia (NO) versus cells grown with intermittent exposure to hypoxia (n = 4 independent experiments). Cells treated with etopside (ETO) were used as positive control. Data are presented as mean ± SEM. P-values determined by one-tailed paired t-test compared to the NO control.
