Figure 7
Anti-PD-1 antibody binding sites in the neutrophil membrane surface. (A) Schematic representation of the double immunofluorescence experiment. (B) Schematic representation of the results: the anti-PD-1 antibody binds to two different sites of the neutrophil membrane surface, PD-1 receptors and fragment crystallizable (Fc)-gamma receptors (FcγR). (C) Representative confocal microscopy images of anti-PD-1 bound to PD-1 receptor (in green) in insolated murine neutrophils. The PD-1 active protein (in red) is bound to anti-PD-1 which is in turn is attached to the neutrophil surface membrane through FcγR. Right panel shows merged images of co-localization (yellow) of both receptors in the neutrophil. Scale bars, 10 µm. Abbreviations: Fab, antigen-binding fragment; FITC, fluorescein isothiocyanate; PE, phycoerythrin.
