Figure 4
Effects of Sprn invalidation on Milk fat globules volume and morphology. (A) (a,a’) Comparative representations of the milk fat globule volumes distribution between FVB/NJ and Sprn0/0 milks. At least 3 individual milks were analyzed per genotype, as described in the Material and Methods section. (A) (b,b’) Comparative representations of 0 to 100 µm3 milk fat globule distribution between FVB/NJ and Sprn0/0 milks. At least 3 individual milks were analyzed per genotype, as described in the Material and Methods section. (B) Immunofluorescence imaging of MFGs from FVB/NJ or Sprn0/0 mice at day 10 of lactation. MFGs were co-stained for for neutral lipids (green) with Bodipy 493/503 and (red) for either cytoplasmic crescents (arrowheads) with acridine orange, membranes with FM4–64, glycoproteins with wheat germ agglutinin (WGA) or free cholesterol with filipin. Bar, 10 µm.
