Figure 3
From: Light-Sheet Fluorescence Microscopy with Scanning Non-diffracting Beams
Numerically simulated LSFM images of non-fluorescent micro-beads embedded in a uniform fluorescent medium, with a scanning Bessel (a), and a scanning 2D Airy (b) light-sheets. (c and d) Present corresponding intensity gradient (modulus) of (a), and (b), respectively. (e) Line profiles of the intensity gradient over the indicated dashed lines in (c and d) along \(x\)-axis. For a comparison a line profile of the intensity gradient modulus corresponding to scanning Gaussian light-sheet is plotted as well. The beads are located at several depths, and the Bessel and 2D Airy beams have identical widths at the middle of the field-of-view, and a comparable number of side lobes.
