Figure 4

Schematic of the experimentally developed LSFM. The collimated laser beam(s) are incident on a SLM, for imprinting a desired phase profile, and then imaged into the focal plane of the illumination objective (IO) by two 4f-systems composed of the lenses L1, L2, L3, and L4. The scan mirror (SM), located at the back focal plane of the IO, provides the beam scanning capability along x–axis, for the generation of scanning light-sheets. The waist of the light-sheets overlap with the middle of the field-of-view (FOV) of an orthogonally oriented detection microscope, comprising a detection objective (DO), an infinity-corrected tube lens (TL), a long-pass filter (F) and a CCD camera. For excitation with 532 nm, a mirror (shown in the dashed box) was placed in front of the second laser.