Figure 7 | Scientific Reports

Figure 7

From: Non-cell autonomous promotion of astrogenesis at late embryonic stages by constitutive YAP activation

Figure 7The alternative text for this image may have been generated using AI.

YAP 5SA expression ectopically produces SMA+ cells. (A, B) E13.5 neural progenitor cells transduced with YAP 5SA retroviruses were cultured in differentiation medium for 2 days, and (A) qPCR analysis of SMA and FN1 genes and (B) coimmunostaining using anti-GFP (green) and anti-SMA (red) antibodies were carried out. Cortical regions of E18.5 brains transduced with YAP 5SA-expressing retroviruses at E13.5 were labeled with (C) anti-GFAP (red) and anti-SMA (blue) antibodies, or (D) anti-GFP (green) antibody together with anti-SMA (red) or anti-FN1 (red) antibodies. Z-stack views at the position of the red line are shown on the right. LV, lateral ventricle. Scale bars, 20 μm for (C), 50 μm for (D), and 100 μm for (B). Student’s t-test was used to determine statistical significance. **P < 0.01, ***P < 0.001; n ≥ 3.

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